Histochemical Demonstration of Protein-Bound Alpha-Acylamido Carboxyl Groups
نویسندگان
چکیده
منابع مشابه
Histochemical Demonstration of Protein-Bound Alpha-Acylamido Carboxyl Groups
A method has been developed to demonstrate the alpha-acylamido carboxyl groups of protein, taking advantage of the fact that acylamido carboxyl groups are converted to ketonic carbonyls by the action of acetic anhydride and absolute pyridine. The method utilizes deparaffinized sections of tissues fixed in a variety of fixatives. Following the conversion of carboxyls to the methyl ketones, the l...
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The specificity of the Barrnett-Seligman method for the histochemical demonstration of alpha-acylamido carboxyl groups (C terminal) of proteins is dependent on the conversion of such groups to ketones by the action of acetic anhydride and absolute pyridine. Studies on model compounds show that the side-chain carboxyl groups also react in the method and that most of the final color developed can...
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Experiments were carried out to assess the susceptibility of normal and inflammatory bowel disease rectal mucus to desulphation and desialation by faecal extracts and by bacterial sialidase. The effects were assessed histochemically using a combined high iron diamine (HID) and alcian blue (AB) stain for sulphomucins and sialomucins. Rectal mucus in biopsies from controls (irritable bowel syndro...
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Histochemical methods for the localization of protein-bound sulphydryl groups were compared in a critical fashion. As far as localization is concerned, several of the methods published were found to be satisfactory. However, from the point of view of approximate visual quantitation, the results are confusing. The two best methods for the histochemical detection of protein-bound sulphydryl group...
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The Paneth cell secretion of the mouse intestine has been reported to be an acid mucopolysaccharide-protein complex; tryptophan as well as alpha acylamido carboxyl, amino, and phenolic groups of protein have been localized to the characteristic granules by means of histochemical staining procedures (13). Sulfhydryl and disulfide groups of protein have also been localized to the granule (14). Th...
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ژورنال
عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology
سال: 1958
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.4.2.169